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Anticancer Res. 2018 Sep;38(9):5079-5086. doi: 10.21873/anticanres.12827.

Trichomonas Vaginalis Inhibits HeLa Cell Growth Through Modulation of Critical Molecules for Cell Proliferation and Apoptosis.

Author information

1
Department of Microbiology, Immunology & Pathology, Des Moines University College of Osteopathic Medicine, Des Moines, IA, U.S.A.
2
Department of Surgery, University of Missouri School of Medicine, Columbia, MO, U.S.A.
3
Department of Respiratory Medicine, the Second People's Hospital of Hefei and Hefei Hospital Affiliated to Anhui Medical University, Hefei, P.R. China.
4
Department of Microbiology, Immunology & Pathology, Des Moines University College of Osteopathic Medicine, Des Moines, IA, U.S.A. yujiang.fang@dmu.edu.

Abstract

BACKGROUND/AIM:

Cervical cancer is one of the deadliest gynecological cancers in USA. The role of Trichomonas Vaginalis (T. Vag) in the etiology or pathogenesis of cervical cancer is still poorly understood and controversial.

MATERIALS AND METHODS:

Clonogenic assay, PCNA staining, TUNEL staining and caspase-3 activity assay were used to investigate the direct in vitro effect of T. Vag on human cervical cancer by using HeLa cells. We further investigated the potential molecular mechanisms using RT-PCR and immunohistochemical staining.

RESULTS:

We found that culture supernatant of T. Vag inhibited growth of HeLa cervical cancer cells and this correlated with up-regulation of p15. We also found that culture supernatant of T. Vag induced apoptosis of HeLa cells and this correlated with up-regulation of Fas, TRAIL and TRAILR1.

CONCLUSION:

Culture supernatant of T. Vag inhibits growth of HeLa cervical cancer cells by inhibition of proliferation and promotion of apoptosis. Our study might be helpful to address the association between the development of cervical cancer and infection of T. Vag.

KEYWORDS:

TRAILR1; Trichomonas Vaginalis; apoptosis; cervical cancer; p15; proliferation

PMID:
30194152
DOI:
10.21873/anticanres.12827
[Indexed for MEDLINE]

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