Format

Send to

Choose Destination
Cancer Genomics Proteomics. 2018 Sep-Oct;15(5):413-420. doi: 10.21873/cgp.20100.

Indirect Down-regulation of Tumor-suppressive let-7 Family MicroRNAs by LMO1 in Neuroblastoma.

Author information

1
Division of Anatomy and Physiology, Okinawa Prefectural College of Nursing, Okinawa, Japan saeki@okinawa-nurs.ac.jp.
2
Statistical Genetics Analysis Division, StaGen Co. Ltd., Tokyo, Japan.
3
Department of Translational Oncology, National Cancer Center Research Institute, Tokyo, Japan.

Abstract

BACKGROUND/AIM:

Overall survival for the high-risk group of neuroblastoma (NB) patients still remains at 40-50%, necessitating the establishment of a curable treatment. LIM domain only 1 (LMO1) gene encoding a transcriptional regulator is an NB-susceptibility gene with a tumor-promoting activity. Previously we conducted chromatin immunoprecipitation and DNA sequencing analyses on NB cell lines and identified 3 protein-coding genes regulated by LMO1. In this study, we extended our analyses to capture microRNA genes directly or indirectly regulated by LMO1.

MATERIALS AND METHODS:

Using microarrays, we conducted a comparative gene expression analysis on an NB cell line SK-N-SH; between the cells with and without LMO1 suppression.

RESULTS:

Overall, 18 microRNAs were identified to be indirectly down-regulated by LMO1 including 7 microRNAs of the let-7 family, whose cell proliferation inhibitory activity was observed.

CONCLUSION:

Target genes of the LMO1-regulated microRNAs and their relevant pathways may be a potential therapeutic target.

KEYWORDS:

LMO1; Neuroblastoma; let-7; microRNA; pediatric cancer

PMID:
30194082
PMCID:
PMC6199578
DOI:
10.21873/cgp.20100
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center