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Anal Chem. 2018 Sep 18;90(18):10650-10653. doi: 10.1021/acs.analchem.8b02699. Epub 2018 Sep 7.

Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS.

Author information

1
Newomics Inc. , 804 Heinz Avenue, Suite 150 , Berkeley , California 94710 , United States.

Abstract

Direct quantitation of proteins in complex biological matrices by mass spectrometry remains a challenge. Here, we describe a novel top-down parallel reaction monitoring-mass spectrometry (PRM-MS) assay, enabled by microflow LC-nanospray MS using a silicon microfluidic LC-MS chip. We demonstrated direct analysis of intact proteins such as somatropin in human plasma, achieving sensitivity (0.1-1.0 fmole) and speed (1-5 min) on par with enzyme-linked immunosorbent assay (ELISA).

PMID:
30192519
PMCID:
PMC6143396
[Available on 2019-09-18]
DOI:
10.1021/acs.analchem.8b02699
[Indexed for MEDLINE]

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