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Int J Legal Med. 2019 Mar;133(2):365-372. doi: 10.1007/s00414-018-1919-0. Epub 2018 Sep 6.

Multiplex analysis of genetic polymorphisms within UGT1A9, a gene involved in phase II of Δ9-THC metabolism.

Author information

1
Institute of Legal Medicine, University of Münster, Röntgenstraße 23, 48149, Münster, Germany.
2
Institute of Legal Medicine, University of Münster, Röntgenstraße 23, 48149, Münster, Germany. Jennifer.schuerenkamp@ukmuenster.de.

Abstract

We present a novel multiplex assay for the simultaneous detection of 12 polymorphisms within the UGT1A9 sequence, which codes for enzymes involved in phase II biotransformation. The assay combines a multiplexed amplification step with single-base extension sequencing. The method described here is fast, cost-effective, and easy-to-use, combining the relevant features of screening methods for research and diagnostics in pharmacogenetics. To validate the assay, we tested reproducibility and sensitivity and analysed allele frequencies of 110 Caucasian individuals. Furthermore, we describe combining genetic information of individuals consuming Cannabis sativa products with respective plasma concentrations of a metabolite.

KEYWORDS:

Biological assay; Cannabis; Delta(9)-THC; Personalised medicine; UDP-glucuronosyltransferase 1A9

PMID:
30191314
DOI:
10.1007/s00414-018-1919-0
[Indexed for MEDLINE]

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