Distribution of Porphyromonas gingivalis fimA and mfa1 fimbrial genotypes in subgingival plaques

Keiji Nagano; Yoshiaki Hasegawa; Yura Iijima; Takeshi Kikuchi; Akio Mitani

doi:10.7717/peerj.5581

Distribution of Porphyromonas gingivalis fimA and mfa1 fimbrial genotypes in subgingival plaques

PeerJ. 2018 Aug 27:6:e5581. doi: 10.7717/peerj.5581. eCollection 2018.

Authors

Keiji Nagano¹, Yoshiaki Hasegawa¹, Yura Iijima¹, Takeshi Kikuchi², Akio Mitani²

Affiliations

¹ Department of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
² Department of Periodontology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.

Abstract

Background: Strains of periodontal disease-associated bacterium Porphyromonas gingivalis have different pathogenicity, which can be attributed to clonal genetic diversity. P. gingivalis typically expresses two types of fimbriae, FimA and Mfa1, which comprise six (I, Ib, II, III, IV, and V) and two (mfa⁵³ and mfa⁷⁰ ) genotypes, respectively. This study was conducted to investigate the distribution of the two fimbrial genotypes of P. gingivalis in clinical specimens.

Methods: Subgingival plaques were collected from 100 participants during periodontal maintenance therapy and examined for P. gingivalis fimbrial genotypes by direct polymerase chain reaction and/or DNA sequencing. We also analyzed the relationship between fimbrial genotypes and clinical parameters of periodontitis recorded at the first medical examination.

Results: Both fimbrial types could be detected in 63 out of 100 samples; among them, fimA genotype II was found in 33 samples (52.4%), in which the mfa⁷⁰ genotype was 1.75 times more prevalent than mfa⁵³ . The total detection rate of fimA genotypes I and Ib was 38.1%; in these samples, the two mfa1 genotypes were observed at a comparable frequency. In two samples positive for fimA III (3.2%), only mfa⁵³ was detected, whereas in four samples positive for fimA IV (6.3%), the two mfa1 genotypes were equally represented, and none of fimA V-positive samples defined the mfa1 genotype. No associations were found between clinical parameters and fimbrial subtype combinations.

Discussion: Both P. gingivalis fimbrial types were detected at various ratios in subgingival plaques, and a tendency for fimA and mfa1 genotype combinations was observed. However, there was no association between P. gingivalis fimbrial genotypes and periodontitis severity.

Keywords: FimA; Fimbriae; Genotype; Mfa1; Periodontitis; Porphyromonas gingivalis.

Grants and funding

This study was supported by the Aichi Health Promotion Foundation and JSPS KAKENHI Grant Number 16K11465 (KN). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.