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Acta Neuropathol Commun. 2018 Sep 5;6(1):87. doi: 10.1186/s40478-018-0584-3.

Unique microglia recovery population revealed by single-cell RNAseq following neurodegeneration.

Author information

1
Institute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany. tuan.leng.tay@uniklinik-freiburg.de.
2
Cluster of Excellence BrainLinks-BrainTools, University of Freiburg, Freiburg, Germany. tuan.leng.tay@uniklinik-freiburg.de.
3
Institute of Biology I, Faculty of Biology, University of Freiburg, Freiburg, Germany. tuan.leng.tay@uniklinik-freiburg.de.
4
Max-Planck-Institute of Immunobiology and Epigenetics, Freiburg, Germany.
5
Institute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
6
Max-Planck-Institute of Immunobiology and Epigenetics, Freiburg, Germany. gruen@ie-freiburg.mpg.de.
7
Institute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany. marco.prinz@uniklinik-freiburg.de.
8
BIOSS Centre for Biological Signaling Studies, University of Freiburg, Freiburg, Germany. marco.prinz@uniklinik-freiburg.de.

Abstract

Microglia are brain immune cells that constantly survey their environment to maintain homeostasis. Enhanced microglial reactivity and proliferation are typical hallmarks of neurodegenerative diseases. Whether specific disease-linked microglial subsets exist during the entire course of neurodegeneration, including the recovery phase, is currently unclear. Taking a single-cell RNA-sequencing approach in a susceptibility gene-free model of nerve injury, we identified a microglial subpopulation that upon acute neurodegeneration shares a conserved gene regulatory profile compared to previously reported chronic and destructive neurodegeneration transgenic mouse models. Our data also revealed rapid shifts in gene regulation that defined microglial subsets at peak and resolution of neurodegeneration. Finally, our discovery of a unique transient microglial subpopulation at the onset of recovery may provide novel targets for modulating microglia-mediated restoration of brain health.

KEYWORDS:

Microglia; Neurodegeneration; Recovery; Single-cell RNA analysis

PMID:
30185219
PMCID:
PMC6123921
DOI:
10.1186/s40478-018-0584-3
[Indexed for MEDLINE]
Free PMC Article

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