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Nat Chem Biol. 2018 Oct;14(10):917-927. doi: 10.1038/s41589-018-0117-1. Epub 2018 Sep 3.

Establishment of the PAR-1 cortical gradient by the aPKC-PRBH circuit.

Author information

1
Temasek Life Sciences Laboratory, Singapore, Republic of Singapore.
2
Department of Biological Sciences, National University of Singapore, Singapore, Republic of Singapore.
3
Mechanobiology Institute, National University of Singapore, Singapore, Republic of Singapore.
4
Department of Biomedical Engineering, National University of Singapore, Singapore, Republic of Singapore.
5
Temasek Life Sciences Laboratory, Singapore, Republic of Singapore. fmotegi@tll.org.sg.
6
Department of Biological Sciences, National University of Singapore, Singapore, Republic of Singapore. fmotegi@tll.org.sg.
7
Mechanobiology Institute, National University of Singapore, Singapore, Republic of Singapore. fmotegi@tll.org.sg.

Abstract

Cell polarity is the asymmetric compartmentalization of cellular components. An opposing gradient of partitioning-defective protein kinases, atypical protein kinase C (aPKC) and PAR-1, at the cell cortex guides diverse asymmetries in the structure of metazoan cells, but the mechanism underlying their spatial patterning remains poorly understood. Here, we show in Caenorhabditis elegans zygotes that the cortical PAR-1 gradient is patterned as a consequence of dual mechanisms: stabilization of cortical dynamics and protection from aPKC-mediated cortical exclusion. Dual control of cortical PAR-1 depends on a physical interaction with the PRBH-domain protein PAR-2. Using a reconstitution approach in heterologous cells, we demonstrate that PAR-1, PAR-2, and polarized Cdc42-PAR-6-aPKC comprise the minimal network sufficient for the establishment of an opposing cortical gradient. Our findings delineate the mechanism governing cortical polarity, in which a circuit consisting of aPKC and the PRBH-domain protein ensures the local recruitment of PAR-1 to a well-defined cortical compartment.

PMID:
30177850
DOI:
10.1038/s41589-018-0117-1
[Indexed for MEDLINE]

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