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J Cyst Fibros. 2019 May;18(3):342-348. doi: 10.1016/j.jcf.2018.07.012. Epub 2018 Sep 1.

Differential expression of genes and receptors in monocytes from patients with cystic fibrosis.

Author information

1
Child Health Research Centre (CHRC), The University of Queensland, Australia.
2
Child Health Research Centre (CHRC), The University of Queensland, Australia; Department of Respiratory and Sleep Medicine, Children's Health Queensland, Brisbane, Australia. Electronic address: p.sly@uq.edu.au.
3
Institute for Molecular Bioscience (IMB) and IMB Centre for Inflammation and Disease Research, The University of Queensland, Australia.
4
QIMR Berghofer Medical Research Institute, Brisbane, Australia; Department of Thoracic Medicine, The Prince Charles Hospital, Brisbane, Australia.
5
Child Health Research Centre (CHRC), The University of Queensland, Australia; Department of Respiratory and Sleep Medicine, Children's Health Queensland, Brisbane, Australia.

Abstract

INTRODUCTION:

We previously reported defective alternative polarization (M2) of macrophages and early expression of classically polarized (M1) macrophage markers in unpolarized monocyte-derived macrophages (MDMs) in patients with cystic fibrosis (CF). The present study assessed whether the mechanism(s) underlying defective macrophage polarization resided in circulating monocytes.

METHODS:

Monocyte subsets (classical, intermediate and non-classical), markers for monocyte activation (CD163) and recruitment (CD195), receptors/genes associated with macrophage differentiation and polarization were analyzed in CF and compared with healthy individuals.

RESULTS:

No differences were observed in the monocyte subsets or in the expression of CD163 or CD195. Expression of the M-CSF receptor, TLR4, γC, IL-4Rα, IL-13Rα1, TIMP-1 and Cox-2 were higher in CF monocytes, albeit at low levels, whereas, LRP1, MMP9, MMP28 were downregulated compared to mooncytes from healthy individuals.

CONCLUSIONS:

Our data suggest that differences in CF monocytes may contribute to the reported CFTR-dependent defect in macrophage differentiation, polarization and function.

KEYWORDS:

Cox-2; Cystic fibrosis; Gamma-chain receptor; IL-13/IL-4 receptor; IRF4; LRP1; MMP28; MMP9; Macrophage polarization; Monocyte-derived macrophages (MDMs); Monocytes; TIMP-1; TLR4

PMID:
30177416
DOI:
10.1016/j.jcf.2018.07.012

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