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Biochem Biophys Res Commun. 2018 Sep 26;504(1):277-282. doi: 10.1016/j.bbrc.2018.08.172. Epub 2018 Aug 31.

miR-125b-5p inhibits breast cancer cell proliferation, migration and invasion by targeting KIAA1522.

Author information

1
Department of Pathology, Xinxiang Medical University, No. 601 Jinsui Avenue, Hongqi District, Xinxiang, 453003, PR China.
2
Cardiothoracic Surgery, The Third Affiliated Hospital of Xinxiang Medical University, East Hualan Avenue, Hongqi District, Xinxiang, 453000, PR China.
3
Department of Pathology, Xinxiang Medical University, No. 601 Jinsui Avenue, Hongqi District, Xinxiang, 453003, PR China. Electronic address: xxmu_lina@163.com.

Abstract

Abnormal gene expression due to the dysregulation of microRNAs (miRNAs) often occurred in the initiation or progression of cancers. The aim of this present study was to investigate the function role of miR-125b-5p in breast cancer (BC). Expression levels of miR-125b-5p were determined by quantitative Real-time PCR. Biological functions of miR-125b-5p in the progression of BC were investigated with a series of in vitro experiments including cell counting kit-8 assay, colony formation assay, wound-healing assay and transwell invasion assay. The target of miR-125b-5p in BC was validated by luciferase activity reporter assay and western blot assay. We found miR-125b-5p expression was significantly reduced in BC cell lines compared to the normal breast epithelial cell line. Functional assays showed that cell proliferation, colony formation ability, cell migration, and cell invasion can be suppressed by miR-125b-5p overexpression. Besides, KIAA1522 was validated as a direct target of miR-125b-5p in BC. Collectively, our study showed that miR-125b-5p functions as a tumor suppressor and regulates BC progression through targeting KIAA1522.

KEYWORDS:

Breast cancer; KIAA1522; Tumor suppressor; miR-125b-5p

PMID:
30177391
DOI:
10.1016/j.bbrc.2018.08.172
[Indexed for MEDLINE]

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