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Front Chem. 2018 Jul 20;6:276. doi: 10.3389/fchem.2018.00276. eCollection 2018.

Extracts of Physalis peruviana Protect Astrocytic Cells Under Oxidative Stress With Rotenone.

Author information

1
Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
2
Departamento de Química, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
3
Departamento de Química e Exatas, Universidade Estadual do Sudoeste da Bahia, Jequié, Brazil.
4
Facultad de Ciencias de la Salud, Universidad San Sebastián, Concepción, Chile.
5
Bay Pines VA Healthcare System, Research and Development, Bay Pines, FL, United States.
6
Institute of Physiologically Active Compounds, Russian Academy of Sciences, Chernogolovka, Russia.
7
GALLY International Biomedical Research Consulting LLC., San Antonio, TX, United States.
8
School of Health Science and Healthcare Administration, University of Atlanta, Johns Creek, GA, United States.
9
Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
10
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
11
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
12
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.

Abstract

The use of medicinal plants to counteract the oxidative damage in neurodegenerative diseases has steadily increased over the last few years. However, the rationale for using these natural compounds and their therapeutic benefit are not well explored. In this study, we evaluated the effect of different Physalis peruviana extracts on astrocytic cells (T98G) subjected to oxidative damage induced by rotenone. Extracts of fresh and dehydrated fruits of the plant with different polarities were prepared and tested in vitro. Our results demonstrated that the ethanolic extract of fresh fruits (EF) and acetone-dehydrated fruit extract (AD) increased cell viability, reduced the formation of reactive oxygen species (ROS) and preserved mitochondrial membrane potential. In contrast, we observed a significant reduction in mitochondrial mass when rotenone-treated cells were co-treated with EF and AD. These effects were accompanied by a reduction in the percentage of cells with fragmented/condensed nuclei and increased expression of endogenous antioxidant defense survival proteins such as ERK1/2. In conclusion, our findings suggest that ethanolic and acetone extracts from P. peruviana are potential medicinal plant extracts to overcome oxidative damage induced by neurotoxic compounds.

KEYWORDS:

Physalis peruviana; astrocytes; oxidative stress; phenolics; rotenone; uchuva

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