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Food Chem. 2019 Jan 1;270:429-435. doi: 10.1016/j.foodchem.2018.07.135. Epub 2018 Jul 20.

Stereoselective interactions of lactic acid enantiomers with HSA: Spectroscopy and docking application.

Author information

1
College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China.
2
Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau Taipa, Macau.
3
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
4
Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangdong Provincial Engineering & Technique Research Centre of Food Safety Detection and Risk Assessment, Guangzhou 510642, China.
5
College of Food Science, South China Agricultural University, Guangzhou 510642, China.
6
Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangdong Provincial Engineering & Technique Research Centre of Food Safety Detection and Risk Assessment, Guangzhou 510642, China. Electronic address: hongtao@scau.edu.cn.
7
College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China. Electronic address: yuanxuewen@gdei.edu.cn.

Abstract

Lactic acid enantiomers, normally found in fermented food, are absorbed into the blood and interact with plasma carrier protein human serum albumin (HSA). Unveiling the effect on the function and structure of HSA during chiral interaction can give a better understanding of the different distribution activities of the two enantiomers. Multi-spectroscopic methods and molecular modelling techniques are used to study the interactions between lactic acid enantiomers and HSA. Time-resolved and steady-state fluorescence spectra manifest that the fluorescence quenching mechanism is mainly static in type, due to complex formation. Binding interactions, deduced by thermodynamic calculation, agree with the docking prediction. Docking results and kinetic constants represent chiral-recognizing discriminations consistently. The bindings of lactic acid enantiomers lead to some microenvironmental and slight conformational changes of HSA as shown by circular dichroism (CD), synchronous and three-dimensional fluorescence spectra. This investigation may yield useful information about the possible toxicity risk of lactic acid enantiomers to human health.

KEYWORDS:

Circular dichroism; Docking simulation; Fluorescence spectroscopy; Human serum albumin; Lactic acid isomers

PMID:
30174068
DOI:
10.1016/j.foodchem.2018.07.135
[Indexed for MEDLINE]

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