Format

Send to

Choose Destination
Inflammation. 2019 Feb;42(1):124-134. doi: 10.1007/s10753-018-0878-4.

Study of the Potential Radiomitigator Effect of Quercetin on Human Lymphocytes.

Author information

1
Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil. williams.wns@gmail.com.
2
Departamento de Energia Nuclear, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-540, Brazil. williams.wns@gmail.com.
3
Serviço de Monitoração Ambiental, Centro Regional de Ciências Nucleares do Nordeste, Recife, Pernambuco, 50740-540, Brazil. williams.wns@gmail.com.
4
Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
5
Departamento de Energia Nuclear, Universidade Federal de Pernambuco, Recife, Pernambuco, 50740-540, Brazil.
6
Serviço de Monitoração Ambiental, Centro Regional de Ciências Nucleares do Nordeste, Recife, Pernambuco, 50740-540, Brazil.
7
Laboratório de Imunomodulação e Novas Abordagens Terapêuticas, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.

Abstract

Several substances of synthetic and natural origin have been studied to determine their ability to protect the body from damage caused by ionizing radiation. Among these substances, quercetin has been shown to be a naturally occurring molecule with high radioprotective and radiomitigator potential due to its antioxidant properties. The objective of this work was to ascertain the potential radiomitigator effect of quercetin on chromosome aberration yield in lymphocytes of in vitro-irradiated human peripheral blood. At first, the DPPH (2,2-diphenyl-1-picryl-hydrazyl) radical capture test was performed to determine the antioxidant activity of quercetin and to select the concentrations to be tested. The blood was irradiated at doses of 2.5, 3.5, and 4.5 Gy and lymphocytes were cultured with quercetin at preselected concentrations of 37.5 and 75 μM. Then, the slides were prepared for scoring unstable chromosome aberrations (dicentrics, rings, and fragments). The results showed that the lymphocytes irradiated and later exposed to quercetin presented a lower frequency of chromosomal alterations compared to the control sample which was irradiated and not exposed to quercetin. The results suggest a potential radiomitigator effect of the flavonoid quercetin on human lymphocytes exposed, in vitro, to ionizing radiation. This effect may be related to decrease in the release of cytokines (INF-γ, PGE2, IL-1β, IL6, IL-8) involved in the proinflammatory processes as well as downregulation of NF-kB and reduction of expression TGF-β.

KEYWORDS:

lymphocyte; metaphase; quercetin; radiomitigator

PMID:
30173325
DOI:
10.1007/s10753-018-0878-4
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center