Abstract
The NH2-terminal amino acid sequence of the 100 kilodalton subunit of porcine gastric H+,K+-ATPase has been determined to be YKAENYELYQVELGPGP. Although the NH2-terminal region of this protein is not similar to the same region of the lamb kidney Na+,K+-ATPase catalytic subunit, other regions of these ATPase proteins appear to be homologous. Both monoclonal and polyclonal antibodies raised to lamb kidney Na+,K+-ATPase and its alpha, but not beta, subunit cross-react with the 100 kilodalton protein of H+,K+-ATPase.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / metabolism*
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Amino Acid Sequence
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Animals
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Cross Reactions
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Electrophoresis, Polyacrylamide Gel
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H(+)-K(+)-Exchanging ATPase
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Kidney / enzymology
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Macromolecular Substances
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Molecular Weight
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Sheep
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Sodium-Potassium-Exchanging ATPase / immunology*
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Structure-Activity Relationship
Substances
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Macromolecular Substances
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Adenosine Triphosphatases
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H(+)-K(+)-Exchanging ATPase
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Sodium-Potassium-Exchanging ATPase