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J Cell Biochem. 2018 Dec;119(12):9781-9789. doi: 10.1002/jcb.27296. Epub 2018 Sep 1.

Genomic integrity of ground-state pluripotency.

Author information

1
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
2
Department of Developmental Biology, University of Science and Culture, Tehran, Iran.
3
Department of Genetics/Epigenetics, Saarland University, Saarbrücken, Germany.
4
Regenerative Medicine Center and Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, Utrecht, The Netherlands.
5
Department of Urology, German Cancer Consortium (DKTK) Partner Site Freiburg, German Cancer Research Center (DKFZ), University Medical Center Freiburg, Freiburg, Germany.
6
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Abstract

Pluripotent cells appear to be in a transient state during early development. These cells have the capability to transition into embryonic stem cells (ESCs). It has been reported that mouse pluripotent cells cultivated in chemically defined media sustain the ground state of pluripotency. Because the epigenetic pattern of pluripotent cells reflects their environment, culture under different conditions causes epigenetic changes, which could lead to genomic instability. This study focused on the DNA methylation pattern of repetitive elements (REs) and their activation levels under two ground-state conditions and assessed the genomic integrity of ESCs. We measured the methylation and expression level of REs in different media. The results indicated that although the ground-state conditions show higher REs activity, they did not lead to DNA damage; therefore, the level of genomic instability is lower under the ground-state compared with the conventional condition. Our results indicated that when choosing an optimum condition, different features of the condition must be considered to have epigenetically and genomically stable stem cells.

KEYWORDS:

DNA methylation; embryonic stem cells (ESCs); genomic instability; ground state of pluripotency; repetitive elements (REs)

PMID:
30171711
DOI:
10.1002/jcb.27296
[Indexed for MEDLINE]

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