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BMC Infect Dis. 2018 Aug 31;18(1):443. doi: 10.1186/s12879-018-3337-9.

Epidemiological and genomic characterization of community-acquired Clostridium difficile infections.

Author information

1
Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
2
Department of Medicine, University of Calgary, Calgary, AB, Canada.
3
Calgary Laboratory Services, Calgary, AB, Canada.
4
Department of Veterinary Microbiology, University of Saskatchewan, Regina, Canada.
5
Department of Laboratory Medicine, University of California San Francisco, San Francisco, California, USA.
6
Department of Laboratory Medicine, University of Washington, Seattle, WA, USA.
7
Department of Medicine, Division of Infectious Diseases, University of California San Francisco, San Francisco, CA, USA.
8
Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, AB, Canada. drpillai@ucalgary.ca.
9
Department of Medicine, University of Calgary, Calgary, AB, Canada. drpillai@ucalgary.ca.
10
Calgary Laboratory Services, Calgary, AB, Canada. drpillai@ucalgary.ca.
11
Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, AB, Canada. drpillai@ucalgary.ca.
12
Diagnostic and Scientific Center, Room 1W-416, 9-3535 Research Road NW, Calgary, AB, T2L 2K8, Canada. drpillai@ucalgary.ca.

Abstract

BACKGROUND:

Clostridium difficile infection (CDI) is a major cause of morbidity and mortality in North America and Europe. The aim of this study was to identify epidemiologically-confirmed cases of community-acquired (CA)-CDI in a large North American urban center and analyze isolates using multiple genetic and phenotypic methods.

METHODS:

Seventy-eight patients testing positive for C. difficile from outpatient clinics were further investigated by telephone questionnaire. CA-CDI isolates were characterized by antibiotic susceptibility, pulsed-field gel electrophoresis and whole genome sequencing. CA-CDI was defined as testing positive greater than 12 weeks following discharge or no previous hospital admission in conjunction with positive toxin stool testing.

RESULTS:

51.3% (40/78) of the patients in this study were found to have bona fide CA-CDI. The majority of patients were female (71.8% vs. 28.2%) with 50-59 years of age being most common (21.8%). Common co-morbidities included ulcerative colitis (1/40; 2.5%), Crohn's disease (3/40; 7.5%), celiac disease (2/40; 5.0%) and irritable bowel syndrome (8/40; 20.0%). However, of 40 patients with CA-CDI, 9 (29.0%) had been hospitalized between 3 and 6 months prior and 31 (77.5%) between 6 and 12 months prior. The hypervirulent North American Pulostype (NAP) 1-like (9/40; 22.5%) strain was the most commonly identified pulsotype. Whole genome sequencing of CA-CDI isolates confirmed that NAP 1-like pulsotypes are commonplace in CA-CDI. From a therapeutic perspective, there was universal susceptibility to metronidazole and vancomycin.

CONCLUSIONS:

All CA-CDI cases had some history of hospitalization if the definition were modified to health care facility exposure in the last 12 months and is supported by the genomic analysis. This raises the possibility that even CA-CDI may have nosocomial origins.

PMID:
30170546
PMCID:
PMC6119286
DOI:
10.1186/s12879-018-3337-9
[Indexed for MEDLINE]
Free PMC Article

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