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Mikrochim Acta. 2018 Aug 30;185(9):435. doi: 10.1007/s00604-018-2978-0.

Hydroxyapatite nanoparticle based fluorometric turn-on determination of dipicolinic acid, a biomarker of bacterial spores.

Author information

1
College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Efficient and Clean Utilization of Manganese Resources, Central South University, Changsha, 410083, China.
2
Department of Engineering Management, Hunan College of Finance and Economics, Changsha, 410083, China.
3
College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Efficient and Clean Utilization of Manganese Resources, Central South University, Changsha, 410083, China. yangminghui@csu.edu.cn.

Abstract

Hydroxyapatite nanoparticles (HAP-NPs) were rendered fluorescence by doping with Eu(III) ion. The resulting fluorescent NPs are shown to be viable probes for sensitive and selective determination of dipicolinic acid (DPA), a major constituent of bacterial spores as used in bioterrorism. It is found that the addition of DPA to solutions of such HAP-NPs result in an enhancement of fluorescence due to the coordination of DPA with the Eu(III) dopant. The assay allows DPA to be detected in the 0.1 to 40 μM concentration range and with a 77 nM detection limit. The assay was applied to the detection of spores of Bacillus subtilis. The attractive properties of the probe make it a promising candidate for used in rapid detection of pathogenic bacterial spores. Graphical abstract Fluorescent hydroxyapatite nanoparticles (HAP-NPs) are shown to be a viable probe for detection of dipicolinic acid, a major constituent of bacterial spores. The red asterisks represent the fluorescence intensity of the HAP-NPs.

KEYWORDS:

Bacillus subtilis; Bacterial spores; Dipicolinic acid; Eu(III) ion; Fluorescent probe

PMID:
30167800
DOI:
10.1007/s00604-018-2978-0
[Indexed for MEDLINE]

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