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Sci Rep. 2018 Aug 30;8(1):13088. doi: 10.1038/s41598-018-31280-1.

Effects of sustained daily latanoprost application on anterior chamber anatomy and physiology in mice.

Author information

1
Center for Prevention and Treatment of Visual Loss Iowa City Veterans Administration Medical Center, Iowa City, IA, USA.
2
Department of Ophthalmology and Visual Science, University of Iowa, Iowa City, IA, USA.
3
Electrical and Computer Engineering, University of Iowa, Iowa City, IA, USA.
4
Miami Veterans Administration Medical Center and Bascom Palmer Institute, University of Miami, Miami, FL, USA.
5
Center for Prevention and Treatment of Visual Loss Iowa City Veterans Administration Medical Center, Iowa City, IA, USA. michael-g-anderson@uiowa.edu.
6
Department of Ophthalmology and Visual Science, University of Iowa, Iowa City, IA, USA. michael-g-anderson@uiowa.edu.
7
Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA, USA. michael-g-anderson@uiowa.edu.

Abstract

Latanoprost is a common glaucoma medication. Here, we study longitudinal effects of sustained latanoprost treatment on intraocular pressure (IOP) in C57BL/6J mice, as well as two potential side-effects, changes in iris pigmentation and central corneal thickness (CCT). Male C57BL/6J mice were treated daily for 16 weeks with latanoprost. Control mice were treated on the same schedule with the preservative used with latanoprost, benzalkonium chloride (BAK), or handled, without ocular treatments. IOP and CCT were studied at pre-treatment, 2 "early" time points, and 2 "late" time points; slit-lamp analysis performed at a late time point; and expression of corneal and iridial candidate genes analyzed at the end of the experiment. Latanoprost lowered IOP short, but not long-term. Sustained application of BAK consistently resulted in significant corneal thinning, whereas sustained treatment with latanoprost resulted in smaller and less consistent changes. Neither treatment affected iris pigmentation, corneal matrix metalloprotease expression or iridial pigment-related genes expression. In summary, latanoprost initially lowered IOP in C57BL/6J mice, but became less effective with sustained treatment, likely due to physiological adaptation. These results identify a new resource for studying changes in responsiveness associated with long-term treatment with latanoprost and highlight detrimental effects of commonly used preservative BAK.

PMID:
30166564
PMCID:
PMC6117323
DOI:
10.1038/s41598-018-31280-1
[Indexed for MEDLINE]
Free PMC Article

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