Format

Send to

Choose Destination
Science. 2018 Aug 31;361(6405):866-869. doi: 10.1126/science.aat5011.

CRISPR-Cas guides the future of genetic engineering.

Knott GJ1, Doudna JA2,3,4,5,6.

Author information

1
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
2
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. doudna@berkeley.edu.
3
Department of Chemistry, University of California, Berkeley, CA, USA.
4
Howard Hughes Medical Institute, University of California, Berkeley, CA, USA.
5
Innovative Genomics Institute, University of California, Berkeley, CA, USA.
6
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

Abstract

The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to manipulate the genomes of cultured cells, animals, and plants, accelerating the pace of fundamental research and enabling clinical and agricultural breakthroughs. We describe the basic mechanisms that set the CRISPR-Cas toolkit apart from other programmable gene-editing technologies, highlighting the diverse and naturally evolved systems now functionalized as biotechnologies. We discuss the rapidly evolving landscape of CRISPR-Cas applications, from gene editing to transcriptional regulation, imaging, and diagnostics. Continuing functional dissection and an expanding landscape of applications position CRISPR-Cas tools at the cutting edge of nucleic acid manipulation that is rewriting biology.

PMID:
30166482
PMCID:
PMC6455913
DOI:
10.1126/science.aat5011
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center