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Nature. 1986 Jul 17-23;322(6076):228-32.

Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria.

Abstract

Methotrexate, a folate antagonist, blocks import into mitochondria of mouse dihydrofolate reductase fused to a mitochondrial presequence. Methotrexate does not mask the presequence, but stabilizes the dihydrofolate reductase moiety. It does not inhibit import of the authentic precursor from which the presequence is derived. This suggests that dihydrofolate reductase must at least partly unfold in order to be transported across mitochondrial membranes.

PMID:
3016548
DOI:
10.1038/322228a0
[Indexed for MEDLINE]

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