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Parasitol Int. 2018 Dec;67(6):816-823. doi: 10.1016/j.parint.2018.08.008. Epub 2018 Aug 28.

Pfcrt genotypes and related microsatellite DNA polymorphisms on Plasmodium falciparum differed among populations in the Greater Mekong Subregion.

Author information

1
Department of Tropical Medicine and Malaria, National Center for Global Health and Medicine, Research Institute, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan; Ministry of Health, Institut Pasteur du Laos, Vientiane, Laos; SATREPS Project for Parasitic Diseases, Vientiane, Laos.
2
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
3
Department of Tropical Hygiene, Mahidol University, Bangkok, Thailand.
4
Molecular Biology Department, Parasitology and Entomology, National Institute of Malariology, Hanoi, Viet Nam.
5
Department of Protozoology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
6
National Centre for Parasitology, Entomology and Malaria Control, Ministry of Health, Phnom Penh, Cambodia.
7
Department of Community and Global Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
8
SATREPS Project for Parasitic Diseases, Vientiane, Laos; Department of Community and Global Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
9
Division of Cellular and Molecular Immunology, Center of Molecular Biosciences, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
10
Department of Global Health, School of Health Sciences, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan.
11
Department of Parasitology and Immunopathoetiology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
12
Center of Malariology, Parasitology and Entomology, Ministry of Health, Vientiane, Laos.
13
SATREPS Project for Parasitic Diseases, Vientiane, Laos; Center of Malariology, Parasitology and Entomology, Ministry of Health, Vientiane, Laos.
14
Ministry of Health, Institut Pasteur du Laos, Vientiane, Laos; SATREPS Project for Parasitic Diseases, Vientiane, Laos.
15
Department of Tropical Medicine and Malaria, National Center for Global Health and Medicine, Research Institute, 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan; Ministry of Health, Institut Pasteur du Laos, Vientiane, Laos; SATREPS Project for Parasitic Diseases, Vientiane, Laos. Electronic address: kano@ri.ncgm.go.jp.

Abstract

Malaria morbidity and mortality have decreased gradually in the Greater Mekong Subregion (GMS). Presently, WHO sets a goal to eliminate malaria by 2030 in the GMS. However, drug-resistant malaria has been reported from several endemic areas. To achieve the goal of elimination, the status of the emergence and spread of drug resistance should be monitored. In this study, the genotype of the Plasmodium falciparum chloroquine (CQ) resistance transporter gene (pfcrt) and 6 microsatellite DNA loci flanking the gene were examined. P. falciparum isolates (n = 136) was collected from malaria patients in Thailand (n = 50, 2002-2005), Vietnam (n = 39, 2004), Laos (n = 15, 2007) and Cambodia (n = 32, 2009). Amino acid sequences at codons 72-76 on the gene were determined. All of the isolates from Thailand were CQ-resistant (CVIET), as were all of the isolates from Cambodia (CVIET, CVIDT). Thirteen of the 15 isolates (87%) from Laos were CQ-resistant (CVIET, CVIDT), whereas the other 2 (13%) were CQ-susceptible (CVMNK). In contrast, 27 of the 39 isolates (69%) from Vietnam were CQ-susceptible (CVMNK), whereas the other 12 (31%) were CQ-resistant (CVIET, CVIDT, CVMDT) or mixed (CVMNK/CVIDT). The mean of expected heterozygosity of the microsatellite loci was 0.444 in the Thai population, 0.482 in the Cambodian population, and 0.734 in the Vietnamese population. Genetic diversity in the Thai population was significantly lower than that in the Vietnamese population. These results suggested that chloroquine selective pressure on P. falciparum populations is heterogeneous in the GMS. Therefore, further examination to understand the mechanisms behind the emergence and spread of drug-resistant malaria are needed.

PMID:
30165262
DOI:
10.1016/j.parint.2018.08.008
[Indexed for MEDLINE]

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