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J Mol Diagn. 2018 Nov;20(6):849-858. doi: 10.1016/j.jmoldx.2018.07.004. Epub 2018 Aug 28.

A Head-to-Head Analytical Comparison of Cobas 4800 HPV, PapilloCheck HPV Screening, and LMNX Genotyping Kit HPV GP for Detection of Human Papillomavirus DNA in Cervical and Cervicovaginal Swabs.

Author information

1
Institute of Molecular and Translational Medicine, Palacky University Olomouc, Olomouc, Czech Republic; Cancer Research Czech Republic, Olomouc, Czech Republic.
2
Fertimed Ltd., Olomouc, Czech Republic; Department of Biology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.
3
Fertimed Ltd., Olomouc, Czech Republic; Department of Obstetrics and Gynecology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.
4
Arleta IVF Ltd., Kostelec nad Orlici, Czech Republic.
5
Department of Obstetrics and Gynecology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.
6
Institute of Molecular and Translational Medicine, Palacky University Olomouc, Olomouc, Czech Republic; Cancer Research Czech Republic, Olomouc, Czech Republic. Electronic address: marian.hajduch@upol.cz.

Abstract

High-risk human papillomavirus (hrHPV) infection is a cause of cervical cancer development. The addition of hrHPV testing to cervical cancer screening and monitoring of cervical intraepithelial neoplasia treatment improves the efficacy of screening and treatment, respectively. Self-sampling for hrHPV testing seems a promising tool for increasing patient participation in cervical cancer screening. In this project, 1198 cervical swabs obtained by physicians and 176 cervicovaginal swabs obtained by self-sampling (not collected in parallel) were analyzed for the presence of 14 hrHPV genotypes using three commercially available assays in comparison. HPV DNA was detected in 21.2% of all samples (21% of cervical swabs and 22.7% of cervicovaginal swabs). The cobas 4800 HPV Test was the most sensitive (0.983) and specific (0.992) for hrHPV detection overall. The PapilloCheck HPV-Screening and LMNX Genotyping Kit HPV GP had comparable specificity with that of the cobas (0.989 and 0.955, respectively), but lesser sensitivity (0.897 and 0.909, respectively). In physician-obtained cervical swabs, the cobas showed the highest sensitivity and specificity (0.980 and 0.994, respectively) for hrHPV detection, whereas in cervicovaginal swabs, the cobas had the highest sensitivity (1.00), but the PapilloCheck had the highest specificity (0.993). In conclusion, all of the detection methods evaluated were highly sensitive and specific for hrHPV detection from both clinician-collected cervical swabs and self-sampled cervicovaginal swabs.

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