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J Oral Maxillofac Pathol. 2018 May-Aug;22(2):283. doi: 10.4103/jomfp.JOMFP_189_16.

Comparison of efficacy of local anesthetic solution, distilled water and normal saline as emergency fixatives.

Author information

1
Department of Oral Basic and Clinical Sciences, College of Dentistry, Buraydah, KSA.
2
Department of Oral Pathology, People's College of Dental Science and Research Centre, Bhopal, Madhya Pradesh, India.
3
Department of Oral Pathology and Microbiology, RKDF, Bhopal, Madhya Pradesh, India.
4
Department of Oral Maxillofacial Surgery, Ayder Referral Hospital - College of Health Sciences, Mekelle University, Ethiopia.

Abstract

Context:

Adequate tissue fixation is fundamental to good quality histological sections. Owing to undesirable effects of 10% buffered formalin, its availability in clinics is questionable. Thus, the present study was conducted with a novel approach to fixation, together with the scope of finding fixative properties of more commonly used reagents available at the clinics.

Aims:

The present study was aimed to compare the efficacy of local anesthetic solution, normal saline (NS) and distilled water (DW) with that of 10% neutral-buffered formalin.

Settings and Design:

It is a single-blinded study where histological assessment of fixation was done to assert if the tissues procured were sufficient or insufficient for the clinical diagnosis with/without any problems.

Subjects and Methods:

Forty soft-tissue specimens obtained from 2 goat tongue were used. Tissues each were directly immersed in local anesthesia, DW, NS solution and formalin for 12 and 24 h each and labeled as Group I and Group II, respectively. The sections were evaluated for staining quality and were subjected to statistical analysis.

Statistical Analysis Used:

Kruskal-Wallis test was employed to assess the differences in histological quality scores. Comparison between the tissues of the two groups was estimated with Mann-Whitney U-test. Kappa Statistic was used to measure the interobserver variation.

Results:

There was a significant difference (P ≤ 0.05) in the efficacy of all the three emergency fixatives.

Conclusions:

On the basis of the results obtained, local anesthetic solution can be used as an emergency fixative.

KEYWORDS:

10% neutral-buffered formalin; distilled water; emergency fixatives; local anesthetic solution; normal saline

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