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Mol Brain. 2018 Aug 29;11(1):47. doi: 10.1186/s13041-018-0389-0.

Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force.

Jia JM1,2, Peng C3, Wang Y4,5, Zheng J3, Ge WP6,7,8,9,10.

Author information

1
Children's Research Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA. jiajiemin@wias.org.cn.
2
School of Life Sciences, Westlake University, Hangzhou, China. jiajiemin@wias.org.cn.
3
Department of Chemistry and Biochemistry, University of Texas at Dallas, Richardson, TX, USA.
4
Children's Research Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA.
5
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
6
Children's Research Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA. woo-ping.ge@utsouthwestern.edu.
7
Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX, USA. woo-ping.ge@utsouthwestern.edu.
8
Departments of Neuroscience, University of Texas Southwestern Medical Center, Dallas, TX, USA. woo-ping.ge@utsouthwestern.edu.
9
Department of Neurology and Neurtherapeutics, University of Texas Southwestern Medical Center, Dallas, TX, USA. woo-ping.ge@utsouthwestern.edu.
10
Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, USA. woo-ping.ge@utsouthwestern.edu.

Abstract

Ischemic perinatal stroke (IPS) is common, resulting in significant mortality and morbidity. In such cases, the incidence of unilateral arterial cerebral infarction is often occluded in the middle cerebral artery (MCA), leading to focal ischemia. In adult rodents, blockage of MCA is the most frequently used strategy for ischemic stroke study. However, modeling MCA occlusion (MCAo) in postnatal day 0-7 (P0-7) mouse pups for IPS study has not been accomplished. Here we occluded the dMCA by inducing the accumulation of magnetic particles (MPs) administered through the superficial temporal vein of mice between P0 and P7, which we called neonatal or perinatal SIMPLE (Stroke Induced with Magnetic Particles). SIMPLE produced either permanent or transient occlusion in the dMCA of perinatal and neonatal mice. Permanent MCA occlusion with SIMPLE resulted in cerebral infarction and neuronal death in the brain. SIMPLE can also be used to reliably produce focal ischemic stroke in neonatal or perinatal mouse brains. As a result, SIMPLE allows the modeling of IPS or focal ischemic stroke for further mechanistic studies in mice, with particular utility for mimicking transient focal ischemia in human pre-term babies, which for the first time here has been accomplished in mice.

KEYWORDS:

Distal MCA; Ischemic neonatal stroke; Ischemic perinatal stroke; Magnetic force; Magnetic nanoparticles; SIMPLE

PMID:
30157965
PMCID:
PMC6114863
DOI:
10.1186/s13041-018-0389-0
[Indexed for MEDLINE]
Free PMC Article

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