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Int J Anal Chem. 2018 Aug 1;2018:8195784. doi: 10.1155/2018/8195784. eCollection 2018.

Determination of Total Flavonoids Contents and Antioxidant Activity of Ginkgo biloba Leaf by Near-Infrared Reflectance Method.

Zhao LJ1,2, Liu W3, Xiong SH1,2, Tang J1,2, Lou ZH4, Xie MX2, Xia BH1,2, Lin LM1,2, Liao DF1,2.

Author information

1
College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
2
Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China.
3
School of Management of Information Engineering, Hunan University of Chinese Medicine, Changsha 410208, China.
4
Institute of Material Medical, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.

Abstract

Background:

Total flavonoids content (TFC) is one of the most important quality indexes of Ginkgo biloba leaf, and it is concerned with total antioxidant activity. Near-infrared spectroscopy (NIR) method has showed its advantages in fast, accurate, qualitative, and quantitative analysis of various components in many quality control researches. In this study, a calibration model was built by partial least squares regression (PLSR) coupling with NIR spectrum to quantitatively analyze the TFC and total antioxidant activity of Ginkgo biloba leaf.

Results:

During the model establishing, some spectrum pretreatment and outlier diagnosis methods were optimized to establish the final model. The coefficients of determination (R2 ) for TFC and total antioxidant activity prediction were 0.8863 and 0.8486, respectively; and the root mean square errors of prediction (RMSEP) were 2.203 mg/g and 0.2211 mM/g, respectively.

Conclusion:

These results showed that NIR method combined with chemometrics is suitable for quantitative analysis of main components and their activities and might be applied to quality control of relevant products.

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