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Int J Anal Chem. 2018 Aug 1;2018:8195784. doi: 10.1155/2018/8195784. eCollection 2018.

Determination of Total Flavonoids Contents and Antioxidant Activity of Ginkgo biloba Leaf by Near-Infrared Reflectance Method.

Zhao LJ1,2, Liu W3, Xiong SH1,2, Tang J1,2, Lou ZH4, Xie MX2, Xia BH1,2, Lin LM1,2, Liao DF1,2.

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College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China.
School of Management of Information Engineering, Hunan University of Chinese Medicine, Changsha 410208, China.
Institute of Material Medical, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.



Total flavonoids content (TFC) is one of the most important quality indexes of Ginkgo biloba leaf, and it is concerned with total antioxidant activity. Near-infrared spectroscopy (NIR) method has showed its advantages in fast, accurate, qualitative, and quantitative analysis of various components in many quality control researches. In this study, a calibration model was built by partial least squares regression (PLSR) coupling with NIR spectrum to quantitatively analyze the TFC and total antioxidant activity of Ginkgo biloba leaf.


During the model establishing, some spectrum pretreatment and outlier diagnosis methods were optimized to establish the final model. The coefficients of determination (R2 ) for TFC and total antioxidant activity prediction were 0.8863 and 0.8486, respectively; and the root mean square errors of prediction (RMSEP) were 2.203 mg/g and 0.2211 mM/g, respectively.


These results showed that NIR method combined with chemometrics is suitable for quantitative analysis of main components and their activities and might be applied to quality control of relevant products.

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