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J Dent. 2018 Nov;78:91-99. doi: 10.1016/j.jdent.2018.08.011. Epub 2018 Aug 25.

Protein-repelling adhesive resin containing calcium phosphate nanoparticles with repeated ion-recharge and re-releases.

Author information

1
Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD, USA; Department of Substitutive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Saudi Arabia.
2
Department of Endodontics, Dental College of Georgia, Augusta University, Augusta, GA, USA.
3
Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD, USA.
4
Volpe Research Center, American Dental Association Foundation, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA.
5
Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD, USA; Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China. Electronic address: dentist.x@163.com.
6
Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD, USA; Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD, 21201, USA; Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD, 21201, USA. Electronic address: hxu@umaryland.edu.

Abstract

OBJECTIVES:

The objectives were to develop a calcium (Ca) and phosphate (P) ion-rechargeable and protein-repellent adhesive containing nanoparticles of amorphous calcium phosphate (NACP) and 2-methacryloyloxyethyl phosphorylcholine (MPC), and investigate the MPC effects on ion recharge and re-releases for the first time.

METHODS:

Pyromellitic glycerol dimethacrylate and ethoxylated bisphenol-A dimethacrylate were used to fabricate adhesive PEHB. Six adhesives were tested: (1) Scotchbond (SBMP); (2) PEHB, (3) PEHB + 20%NACP; (4) PEHB + 30%NACP; (5) PEHB + 20%NACP+3%MPC; (6) PEHB + 30%NACP+3%MPC. Dentin shear bond strength, Ca/P ion release, recharge and re-release, and protein adsorption were measured. A microcosm biofilm model was tested for lactic-acid production and colony-forming units (CFU).

RESULTS:

Adding NACP + MPC did not negatively affect dentin bond strength (p > 0.1). With increasing the number of recharge/re-release cycles, the Ca/P ion re-release reached similarly higher levels (p > 0.1), indicating long-term remineralization capability. One recharge enabled the adhesives to have continued re-releases for 21 days. Incorporation of 3% MPC yielded 10-fold decrease in protein adsorption, and 1-2 log decrease in biofilm CFU.

CONCLUSIONS:

The new rechargeable adhesive with MPC + 30%NACP greatly reduced protein adsorption, biofilm growth and lactic acid. Incorporation of MPC did not compromise the excellent Ca/P ion release, rechargeability, and dentin bond strength.

CLINICAL SIGNIFICANCE:

Novel bioactive adhesive containing MPC + NACP is promising to repel proteins and bacteria, and inhibit secondary caries at the restoration margins. The method of NACP + MPC to combine CaP-recharge and protein-repellency is applicable to the development of a new generation of materials including composites and cements to suppress oral biofilms and plaque formation and protect tooth structures.

KEYWORDS:

Calcium phosphate nanoparticles; Dental adhesive; Dentin bonding; Ion recharge; Protein adsorption; Secondary caries

PMID:
30153499
DOI:
10.1016/j.jdent.2018.08.011
[Indexed for MEDLINE]

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