Format

Send to

Choose Destination
Methods Mol Biol. 2018;1836:303-327. doi: 10.1007/978-1-4939-8678-1_15.

Live Imaging of Influenza Viral Ribonucleoproteins Using Light-Sheet Microscopy.

Author information

1
Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
2
Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Lakdawala@pitt.edu.

Abstract

Influenza viruses exhibit a complex life cycle that is still poorly understood. It involves independent replication of each of the eight segments that make up its genome and subsequent coordinated assembly as they egress from the host cell. Fast, time-resolved volumetric live cell imaging offers a powerful tool for understanding the various host mechanisms hijacked by the virus. Here, we describe the methods necessary for generating influenza viruses that carry a fluorescently tagged polymerase complex, infection of biologically relevant cells with these viruses, and finally protocols for live cell imaging and analysis.

KEYWORDS:

Fluorescence imaging; Fluorescence microscopy; Fluorescent influenza virus; Fluorescent polymerase; Influenza genome; Light-sheet microscopy; Live cell imaging; Particle tracking

PMID:
30151580
DOI:
10.1007/978-1-4939-8678-1_15
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center