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Int J Mol Sci. 2018 Aug 24;19(9). pii: E2503. doi: 10.3390/ijms19092503.

Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression.

Author information

1
Traditional Food Research Group, Korea Food Research Institute, Wanju-gun 55365, Jeollabuk-do, Korea. Han.Ah-ram@kfri.re.kr.
2
Traditional Food Research Group, Korea Food Research Institute, Wanju-gun 55365, Jeollabuk-do, Korea. tglim@kfri.re.kr.
3
Traditional Food Research Group, Korea Food Research Institute, Wanju-gun 55365, Jeollabuk-do, Korea. Song.Young-ran@kfri.re.kr.
4
Traditional Food Research Group, Korea Food Research Institute, Wanju-gun 55365, Jeollabuk-do, Korea. jangmi@kfri.re.kr.
5
Traditional Food Research Group, Korea Food Research Institute, Wanju-gun 55365, Jeollabuk-do, Korea. ykrhee@kfri.re.kr.
6
Traditional Food Research Group, Korea Food Research Institute, Wanju-gun 55365, Jeollabuk-do, Korea. honghd@kfri.re.kr.
7
Traditional Food Research Group, Korea Food Research Institute, Wanju-gun 55365, Jeollabuk-do, Korea. Kim.Mi-hyun@kfri.re.kr.
8
Department of Food Science and Technology, Division of Applied Life Sciences (BK21 plus), and Institute of Agriculture and Life Science, Gyeongsang National University, Jinju-si 52828, Gyeongsangnam-do, Korea. hyunjkim@gnu.ac.kr.
9
Traditional Food Research Group, Korea Food Research Institute, Wanju-gun 55365, Jeollabuk-do, Korea. cwcho@kfri.re.kr.

Abstract

Opuntia humifusa is a type of cactus whose fruits have been used in folk medicine for the treatment of several diseases. In the present study, we aimed to determine whether O. humifusa fruit water extract (OHE) has inhibitory effects against solar ultraviolet (sUV)-induced matrix metalloproteinase-1 (MMP-1) expression. In ex vivo human skin, we found that OHE suppressed sUV radiation-induced MMP-1 expression. The inhibitory effect of OHE was confirmed in human dermal fibroblasts. OHE treatment reduced sUV-induced MMP-1 expression by suppressing reactive oxygen species (ROS) generation and phosphorylation of c-Jun, a component of transcription factor activator protein 1 (AP-1). On the other hand, OHE recovered the tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) and type 1 collagen production attenuated by sUV. As upstream signaling pathways for AP-1, MKK4-JNK, MEK-ERK, and MKK3/6-p38 phosphorylation were downregulated by OHE treatment. In addition, OHE exhibited DPPH radical scavenging activity. These findings demonstrate that OHE has a preventive effect against sUV-induced skin damage via suppression of pathways triggered by ROS.

KEYWORDS:

MMP-1; Opuntia humifusa; ROS; photoaging; sUV damage

PMID:
30149526
PMCID:
PMC6163292
DOI:
10.3390/ijms19092503
[Indexed for MEDLINE]
Free PMC Article

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