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Microb Pathog. 2018 Nov;124:250-257. doi: 10.1016/j.micpath.2018.08.052. Epub 2018 Aug 25.

Development and evaluation of in murine model, of an improved live-vaccine candidate against brucellosis from to Brucella melitensis vjbR deletion mutant.

Author information

1
College of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, Henan, China.
2
College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China. Electronic address: allanzhh@sohu.com.
3
College of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
4
First People's Hospital of Shangqiu, Shangqiu, 476000, Henan, China.
5
College of Biology, Agriculture and Forestry, Tongren University, Tongren, 554300, Guizhou, China.

Abstract

Brucellosis is an infectious disease that brings enormous economic burdens for developing countries. The Brucella melitensis (B. melitensis) M5-90 vaccine strain (M5-90) has been used on a large scale in China, but may cause abortions if given to pregnant goats or sheep subcutaneously during the late stages of gestation. Moreover, the vaccine M5-90 cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent M5-90 vaccine is required. In this study, a vjbR mutant of M5-90 (M5-90ΔvjbR) was constructed and overcame these drawbacks. M5-90ΔvjbR strain showed reduced survival capability in murine macrophages (RAW 264.7) and BALB/c mice and induced high protective immunity in mice. In addition, M5-90ΔvjbR induced an anti-Brucella-specific immunoglobulin G (IgG) response and stimulated the expression of gamma interferon (INF-γ) and interleukin-4 (IL-4) in vaccinated mice. Furthermore, M5-90ΔvjbR induced IgG response and stimulated the secretion of IFN-γ and IL-4 in immunized sheep. Moreover, the VjbR antigen allowed serological differentiation between infected and vaccinated animals. These results suggest that M5-90ΔvjbR is an ideal live attenuated and efficacious live vaccine candidate against B. melitensis 16 M infection.

KEYWORDS:

B. melitensis M5-90; Live attenuated vaccines; vjbR

PMID:
30149131
DOI:
10.1016/j.micpath.2018.08.052
[Indexed for MEDLINE]

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