Accelerated orthodontic tooth movement is currently the subject of numerous scientific studies that aimed to improve the duration of orthodontic treatment. Several approaches had been performed in order to reduce the period in which a patient is subjected to orthodontic treatment. This are biological, surgical and physical approaches. Aim: This study has two purposes: first to assess the influence of local administration of vitamin D3 on orthodontic tooth movement and second to evaluate if there is any secondary effect of locally administration of vitamin D3 on dental roots. Material and methods: Every dental arch has been divided in two: one control quadrant and one experimental quadrant. The control canine received only conventional orthodontic treatment in comparison with the experimental canine who benefited from the association between orthodontic therapy and intraligamentary administration of vitamin D3. Results: 6 dental arches were studied. The average rate of tooth movement was greater in experimental canines compared to control canines. We recorded an average of 70 % more tooth movement for the experimental teeth compared to control ones. The differences between the two quadrants (control and experimental) are statistically significant. Conclusions: Locally administration of vitamin D3 seemed to increase the rate of orthodontic tooth movement. No root resorption was found three months after the first administration of vitamin D3 evaluated on cone-beam CT exam (cone-beam computed tomography).