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Nat Commun. 2018 Aug 24;9(1):3420. doi: 10.1038/s41467-018-05912-z.

Lamin B1 mapping reveals the existence of dynamic and functional euchromatin lamin B1 domains.

Author information

1
Vall d'Hebron Institute of Oncology, 08035, Barcelona, Spain.
2
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona, Spain.
3
Structural Genomic Group, CNAG-CRG, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Baldiri Reixac 4, Barcelona, Spain.
4
Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), 08003, Barcelona, Spain.
5
Institute of Epigenetics and Stem Cells, D-81377, München, Germany.
6
Servei d'Anàlisi de Microarrays Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain.
7
Programa de Recerca en Càncer, Institut Hospital del Mar d'Investigacions Mèdiques, Barcelona, Spain.
8
ICREA, Pg. Lluis Companys 23, Barcelona, Spain.
9
Vall d'Hebron Institute of Oncology, 08035, Barcelona, Spain. speiro@vhio.net.

Abstract

Lamins (A/C and B) are major constituents of the nuclear lamina (NL). Structurally conserved lamina-associated domains (LADs) are formed by genomic regions that contact the NL. Lamins are also found in the nucleoplasm, with a yet unknown function. Here we map the genome-wide localization of lamin B1 in an euchromatin-enriched fraction of the mouse genome and follow its dynamics during the epithelial-to-mesenchymal transition (EMT). Lamin B1 associates with actively expressed and open euchromatin regions, forming dynamic euchromatin lamin B1-associated domains (eLADs) of about 0.3 Mb. Hi-C data link eLADs to the 3D organization of the mouse genome during EMT and correlate lamin B1 enrichment at topologically associating domain (TAD) borders with increased border strength. Having reduced levels of lamin B1 alters the EMT transcriptional signature and compromises the acquisition of mesenchymal traits. Thus, during EMT, the process of genome reorganization in mouse involves dynamic changes in eLADs.

PMID:
30143639
PMCID:
PMC6109041
DOI:
10.1038/s41467-018-05912-z
[Indexed for MEDLINE]
Free PMC Article

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