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Cell. 2018 Aug 23;174(5):1277-1292.e14. doi: 10.1016/j.cell.2018.06.045.

Stereotypic Immune System Development in Newborn Children.

Author information

1
Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17121 Solna, Sweden.
2
Department of Clinical Science, Intervention and Technology, Karolinska Institutet, 14152 Solna, Sweden; Department of Neonatology, Karolinska University Hospital, 17176 Solna, Sweden.
3
Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17121 Solna, Sweden; Department of Neonatology, Karolinska University Hospital, 17176 Solna, Sweden.
4
Science for Life Laboratory, School of Biotechnology, KTH, Royal Institute of Technology, 17121 Stockholm, Sweden.
5
Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, 17121 Solna, Sweden; Department of Neonatology, Karolinska University Hospital, 17176 Solna, Sweden. Electronic address: petter.brodin@ki.se.

Abstract

Epidemiological data suggest that early life exposures are key determinants of immune-mediated disease later in life. Young children are also particularly susceptible to infections, warranting more analyses of immune system development early in life. Such analyses mostly have been performed in mouse models or human cord blood samples, but these cannot account for the complex environmental exposures influencing human newborns after birth. Here, we performed longitudinal analyses in 100 newborn children, sampled up to 4 times during their first 3 months of life. From 100 μL of blood, we analyze the development of 58 immune cell populations by mass cytometry and 267 plasma proteins by immunoassays, uncovering drastic changes not predictable from cord blood measurements but following a stereotypic pattern. Preterm and term children differ at birth but converge onto a shared trajectory, seemingly driven by microbial interactions and hampered by early gut bacterial dysbiosis.

KEYWORDS:

CyTOF; human immunology; immune system development; immune variation; mass cytometry; neonate; neonatology; newborn immune systems; preterm birth; systems immunology

PMID:
30142345
PMCID:
PMC6108833
DOI:
10.1016/j.cell.2018.06.045
[Indexed for MEDLINE]
Free PMC Article

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