Format

Send to

Choose Destination
Am J Trop Med Hyg. 2018 Oct;99(4):1003-1005. doi: 10.4269/ajtmh.18-0250.

Monitoring and Predicting Net Longevity by Measuring Surface Levels of Insecticide: Implementing a Faster, Cost Effective, Nondestructive, and Field-Ready Alternative to the World Health Organization Cone Test Bioassay.

Author information

1
Medical Entomology Group, School of Medicine, Universidad de Antioquia, Medellin, Antioquia, Colombia.
2
Environmental Health Research Group, National School of Public Health, Universidad de Antioquia, Medellin, Antioquia, Colombia.
3
Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia.

Abstract

An important component of malaria control programs is the ability to assess the effectiveness of the insecticide in insecticide-treated nets (ITNs) during normal usage. The standard technique to measure insecticidal activity is the World Health Organization (WHO) cone test, which in many circumstances, may be difficult to implement. We have evaluated an alternative technique, the colorimetric field test (CFT) on a group of 24-month-old Permanet® 2.0 (Vestergaard-Frandsen, Denmark) nets collected in Colombia. The CFT, which measures surface levels (SL) of deltamethrin is compared with standard high-performance liquid chromatography (HPLC) and the WHO cone test. Effective concentrations of deltamethrin for 80% mortality (EC80) were determined from the CFT and HPLC results. Distribution of insecticide SL after 24 months of use reveal that sampling of the midsection best represents the condition of the entire net. We conclude that the CFT is a practical alternative to the WHO cone test for assessing ITN efficacy.

PMID:
30141397
PMCID:
PMC6159599
[Available on 2019-10-01]
DOI:
10.4269/ajtmh.18-0250

Supplemental Content

Full text links

Icon for Ingenta plc
Loading ...
Support Center