Format

Send to

Choose Destination
Sci Rep. 2018 Aug 23;8(1):12658. doi: 10.1038/s41598-018-30107-3.

Tumour cell invasiveness and response to chemotherapeutics in adipocyte invested 3D engineered anisotropic collagen scaffolds.

Author information

1
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
2
Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
3
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK.
4
Department of Materials Science and Metallurgy, 27 Charles Babbage Road, Cambridge, CB3 0FS, UK.
5
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK. cjw53@cam.ac.uk.

Abstract

Breast cancers are highly heterogeneous and their metastatic potential and response to therapeutic drugs is difficult to predict. A tool that could accurately gauge tumour invasiveness and drug response would provide a valuable addition to the oncologist's arsenal. We have developed a 3-dimensional (3D) culture model that recapitulates the stromal environment of breast cancers by generating anisotropic (directional) collagen scaffolds seeded with adipocytes and culturing tumour fragments therein. Analysis of tumour cell invasion in the presence of various therapeutic drugs, by immunofluorescence microscopy coupled with an optical clearing technique, demonstrated the utility of this approach in determining both the rate and capacity of tumour cells to migrate through the stroma while shedding light also on the mode of migration. Furthermore, the response of different murine mammary tumour types to chemotherapeutic drugs could be readily quantified.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center