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Cell Rep. 2018 Aug 21;24(8):2191-2195.e4. doi: 10.1016/j.celrep.2018.07.057.

Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys.

Author information

1
Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan.
2
Graduate School of Science and Technology, Keio University, Kanagawa, Japan.
3
Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Japan Society for the Promotion of Science, Tokyo, Japan.
4
Research Institute of Environmental Medicine, Nagoya University, Nagoya, Japan.
5
Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Center for Brain Science, Saitama, Japan.
6
Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan.
7
James H. Clark Center for Biomedical Engineering and Sciences, Stanford University, Stanford, CA, USA; CNC Program, Stanford University, Stanford, CA, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
8
Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan; Department of Neuropsychiatry, Keio University School of Medicine, Tokyo, Japan.
9
Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Kanagawa, Japan; Keio Institute of Pure and Applied Sciences (KiPAS), Kanagawa, Japan. Electronic address: ushiba@brain.bio.keio.ac.jp.
10
Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Saitama, Japan. Electronic address: hidokano@a2.keio.jp.

Abstract

To understand brain circuits of cognitive behaviors under natural conditions, we developed techniques for imaging neuronal activities from large neuronal populations in the deep layer cortex of the naturally behaving common marmoset. Animals retrieved food pellets or climbed ladders as a miniature fluorescence microscope monitored hundreds of calcium indicator-expressing cortical neurons in the right primary motor cortex. This technique, which can be adapted to other brain regions, can deepen our understanding of brain circuits by facilitating longitudinal population analyses of neuronal representation associated with cognitive naturalistic behaviors and their pathophysiological processes.

KEYWORDS:

calcium imaging; common marmoset; miniaturized microscope; preferred direction; primary motor cortex

PMID:
30134178
DOI:
10.1016/j.celrep.2018.07.057
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