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J Proteome Res. 2018 Dec 7;17(12):4197-4210. doi: 10.1021/acs.jproteome.8b00387. Epub 2018 Aug 31.

Deciphering the Dark Proteome: Use of the Testis and Characterization of Two Dark Proteins.

Author information

1
Univ Rennes , Inserm, EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail)-UMR S 1085 , F-35042 Rennes cedex, France.
2
Protim , Univ Rennes , F-35042 Rennes , France.
3
H2P2 Core Facility, UMS BioSit , Univ Rennes , Rennes F-35040 , France.
4
Sciex , Phoenix House Lakeside Drive Centre Park , Warrington WA1 1RX , U.K.
5
Unité FIV, Clinique Pasteur , 34, Rue du Moulin à Poudre , F-29000 Brest , France.
6
Sciex , Landwehrstr. 54 , 64293 Darmstadt , Germany.

Abstract

For the C-HPP consortium, dark proteins include not only uPE1, but also missing proteins (MPs, PE2-4), smORFs, proteins from lncRNAs, and products from uncharacterized transcripts. Here, we investigated the expression of dark proteins in the human testis by combining public mRNA and protein expression data for several tissues and performing LC-MS/MS analysis of testis protein extracts. Most uncharacterized proteins are highly expressed in the testis. Thirty could be identified in our data set, of which two were selected for further analyses: (1) A0AOU1RQG5, a putative cancer/testis antigen specifically expressed in the testis, where it accumulates in the cytoplasm of elongated spermatids; and (2) PNMA6E, which is enriched in the testis, where it is found in the germ cell nuclei during most stages of spermatogenesis. Both proteins are coded on Chromosome X. Finally, we studied the expression of other dark proteins, uPE1 and MPs, in a series of human tissues. Most were highly expressed in the testis at both the mRNA and protein levels. The testis appears to be a relevant organ to study the dark proteome, which may have a function related to spermatogenesis and germ cell differentiation. The mass spectrometry proteomics data have been deposited with the ProteomeXchange Consortium under the data set identifier PXD009598.

KEYWORDS:

dark proteins; data mining; human proteome project; immunohistochemistry; missing proteins; testis; uPE1

PMID:
30130116
DOI:
10.1021/acs.jproteome.8b00387
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