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Nat Methods. 2018 Sep;15(9):685-688. doi: 10.1038/s41592-018-0105-0. Epub 2018 Aug 20.

Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging.

Author information

1
Department of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.
2
Max Planck Institute of Biochemistry, Martinsried, Germany.
3
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
4
SomaLogic, Inc., Boulder, CO, USA.
5
Department of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany. jungmann@biochem.mpg.de.
6
Max Planck Institute of Biochemistry, Martinsried, Germany. jungmann@biochem.mpg.de.

Abstract

Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.

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PMID:
30127504
PMCID:
PMC6345375
DOI:
10.1038/s41592-018-0105-0
[Indexed for MEDLINE]
Free PMC Article

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