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J Exp Biol. 2018 Oct 17;221(Pt 20). pii: jeb187518. doi: 10.1242/jeb.187518.

Glyphosate impairs learning in Aedes aegypti mosquito larvae at field-realistic doses.

Author information

1
Institut de Recherche sur la Biologie de l'Insecte UMR 7261, CNRS-Université de Tours, 37200 Tours, France.
2
Institut de Recherche sur la Biologie de l'Insecte UMR 7261, CNRS-Université de Tours, 37200 Tours, France fernando.guerreri@univ-tours.fr.

Abstract

Glyphosate is the most widely used herbicide in the world. Over the past few years, the number of studies revealing deleterious effects of glyphosate on non-target species has been increasing. Here, we studied the impact of glyphosate at field-realistic doses on learning in mosquito larvae (Aedes aegypti). Larvae of A. aegypti live in small bodies of water and perform a stereotyped escape response when a moving object projects its shadow on the water surface. Repeated presentations of an innocuous visual stimulus induce a decrease in response due to habituation, a non-associative form of learning. In this study, different groups of larvae were reared in water containing different concentrations of glyphosate that are commonly found in the field (50 µg l-1, 100 µg l-1, 210 µg l-1 and 2 mg l-1). Larvae reared in a glyphosate solution of 2 mg l-1 (application dose) could complete their development. However, glyphosate at a concentration of 100 µg l-1 impaired habituation. A dose-dependent deleterious effect on learning ability was observed. This protocol opens new avenues to further studies aimed at understanding how glyphosate affects non-target organisms, such as insects. Habituation in mosquito larvae could serve as a parameter for testing the impact of pollutants in the water.

KEYWORDS:

Behaviour; Bio-indicator; Ecotoxicology; Fresh-water; Habituation; Herbicide; Pesticide

PMID:
30127074
DOI:
10.1242/jeb.187518
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Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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