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Diagn Pathol. 2018 Aug 21;13(1):56. doi: 10.1186/s13000-018-0733-9.

MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases.

Author information

1
State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, Fourth Military Medical University, West Road #169, Xi'an, Changle, 710032, China.
2
Student Team 1, Class 3, Fourth Military Medical University, West Road #169, Xi'an, Changle, 710032, China.
3
Department of Pathology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Kongjiang Road #1665, Shanghai, 200092, China.
4
Department of Pathology, Hubei Provincial Cancer hospital, Zhuodaoquan South Road #116, Wuhan, 430079, China.
5
Department of Pathology, Affiliated Foshan hospital, Sun Yet-sen University, Lingnan North Road#81, Foshan, 528000, China.
6
State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, Fourth Military Medical University, West Road #169, Xi'an, Changle, 710032, China. zhwang@fmmu.edu.cn.

Abstract

BACKGROUND:

Myxofibrosarcoma (MFS) is one of the most common soft tissue sarcomas. Previous studies have shown that MET protein overexpressed in MFS patients and can serve as a prognostic factor. The reasons for MET protein overexpression include amplification of the MET gene, which is located on chromosome 7q. Triggered by an index case harboring chromosome 7 polysomy rather than MET gene amplification in myxofibrosarcoma, we investigated chromosome 7 polysomy in more cases.

METHODS:

Immunohistochemistry and fluorescence in situ hybridization (FISH) were performed in 30 MFS cases (including 2 epithelioid variant) to detect the expression of MET protein and gene status.

RESULTS:

MET was overexpressed in 14 cases out of 30, while thirteen cases were in higher FNCLCC grades (Grade 2-3). FISH showed that 11 cases having 3 signals on average of Met and more than 3 signals (Mean: 4.6) of centromere 7q (CEP7q). The MET/CEP7 ratio was about 0.65 on average, suggesting that chromosome 7 polysomy, rather than Met gene amplification, leading to the overexpression of MET protein in MFS. MET overexpression and chromosome 7 polysomy are positively correlated with higher Ki-67 index and higher grade and might have a high risk of local recurrence and metastasis.

CONCLUSIONS:

It might reveals another explain of MET overexpression in myxofibrosarcoma, providing a clue for the therapy of MFS.

KEYWORDS:

Chromosome 7 polysomy; FISH; MET; Myxofibrosarcoma

PMID:
30126419
PMCID:
PMC6102798
DOI:
10.1186/s13000-018-0733-9
[Indexed for MEDLINE]
Free PMC Article

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