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Curr Biol. 2018 Sep 10;28(17):2739-2751.e3. doi: 10.1016/j.cub.2018.06.063. Epub 2018 Aug 16.

Synaptic Transfer between Rod and Cone Pathways Mediated by AII Amacrine Cells in the Mouse Retina.

Author information

1
Synaptic Physiology Section, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892, USA.
2
Department of Biology, University of Maryland, College Park, MD 20742, USA.
3
Circuit Dynamics and Connectivity Unit, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892, USA.
4
Synaptic Physiology Section, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892, USA. Electronic address: diamondj@ninds.nih.gov.

Abstract

To understand computation in a neural circuit requires a complete synaptic connectivity map and a thorough grasp of the information-processing tasks performed by the circuit. Here, we dissect a microcircuit in the mouse retina in which scotopic visual information (i.e., single photon events, luminance, contrast) is encoded by rod bipolar cells (RBCs) and distributed to parallel ON and OFF cone bipolar cell (CBC) circuits via the AII amacrine cell, an inhibitory interneuron. Serial block-face electron microscopy (SBEM) reconstructions indicate that AIIs preferentially connect to one OFF CBC subtype (CBC2); paired whole-cell patch-clamp recordings demonstrate that, depending on the level of network activation, AIIs transmit distinct components of synaptic input from single RBCs to downstream ON and OFF CBCs. These findings highlight specific synaptic and circuit-level features that allow intermediate neurons (e.g., AIIs) within a microcircuit to filter and propagate information to downstream neurons.

KEYWORDS:

adaptation; bipolar; connectomics; gap junction; glycine; rectification; ribbon

PMID:
30122532
PMCID:
PMC6133723
DOI:
10.1016/j.cub.2018.06.063
[Indexed for MEDLINE]
Free PMC Article

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