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Sci Rep. 2018 Aug 17;8(1):12388. doi: 10.1038/s41598-018-30856-1.

Genomic Analysis of the Insect-Killing Fungus Beauveria bassiana JEF-007 as a Biopesticide.

Author information

1
Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, 54596, Korea.
2
Department of Biotechnology and Animal Science, National Ilan University, Ilan, Taiwan.
3
Department of Agricultural Biology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju, 54596, Korea. jskim10@jbnu.ac.kr.
4
Plant Medical Research Center, College of Agricultural and Life Sciences, Chonbuk National University, Jenoju, 54596, Korea. jskim10@jbnu.ac.kr.

Abstract

Insect-killing fungi have high potential in pest management. A deeper insight into the fungal genes at the whole genome level is necessary to understand the inter-species or intra-species genetic diversity of fungal genes, and to select excellent isolates. In this work, we conducted a whole genome sequencing of Beauveria bassiana (Bb) JEF-007 and characterized pathogenesis-related features and compared with other isolates including Bb ARSEF2860. A large number of Bb JEF-007 genes showed high identity with Bb ARSEF2860, but some genes showed moderate or low identity. The two Bb isolates showed a significant difference in vegetative growth, antibiotic-susceptibility, and virulence against Tenebrio molitor larvae. When highly identical genes between the two Bb isolates were subjected to real-time PCR, their transcription levels were different, particularly in heat shock protein 30 (hsp30) gene which is related to conidial thermotolerance. In several B. bassiana isolates, chitinases and trypsin-like protease genes involved in pathogenesis were highly conserved, but other genes showed noticeable sequence variation within the same species. Given the transcriptional and genetic diversity in B. bassiana, a selection of virulent isolates with industrial advantages is a pre-requisite, and this genetic approach could support the development of excellent biopesticides with intellectual property protection.

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