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Sci Rep. 2018 Aug 17;8(1):12407. doi: 10.1038/s41598-018-30530-6.

Hsa-miR-10a-5p downregulation in mutant UQCRB-expressing cells promotes the cholesterol biosynthesis pathway.

Author information

1
Chemical Genomics Global Research Lab., Department of Biotechnology, College of Life Science & Biotechnology, Yonsei University, Seoul, 120-749, Republic of Korea.
2
Ewha Research Center for Systems Biology, Division of Molecular & Life Sciences, Ewha Womans University, Seoul, Republic of Korea.
3
Department of Biomedical Science, Hallym University, Chuncheon, Gangwon-do, Republic of Korea.
4
Chemical Genomics Global Research Lab., Department of Biotechnology, College of Life Science & Biotechnology, Yonsei University, Seoul, 120-749, Republic of Korea. kwonhj@yonsei.ac.kr.
5
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, 120-752, Republic of Korea. kwonhj@yonsei.ac.kr.

Abstract

Ubiquinol cytochrome c reductase binding protein (UQCRB) is known to play crucial roles in the development of various types of diseases. However, the link between UQCRB and microRNAs remains unknown. In the present study, we performed microRNA sequencing of mutant UQCRB-expressing stable cell lines that exhibited pro-oncogenic activities caused by expression of the mutant UQCRB gene. Results showed that hsa-miR-10a-5p was significantly downregulated in the mutant UQCRB-expressing cell lines. Furthermore, mRNA sequencing and gene ontology analysis of differentially expressed genes (DEGs) revealed that the cholesterol biosynthesis pathway might be activation by mutant UQCRB expression. Moreover, inhibition of cholesterol synthesis in mutant UQCRB-expressing cells via treatment with the specific inhibitors suppressed the cell proliferation. Transfection with a hsa-miR-10a-5p mimic validated that lanosterol synthase (LSS) is a target of hsa-miR-10a-5p. In addition, hsa-miR-10a-5p was found to be downregulated in liver cancer cell lines overexpressing UQCRB. Taken together, our findings highlighted the potential use of hsa-miR-10a-5p as a biomarker for UQCRB related diseases.

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