Format

Send to

Choose Destination
J Integr Med. 2018 Sep;16(5):350-357. doi: 10.1016/j.joim.2018.07.005. Epub 2018 Jul 29.

Antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava leaf extracts against methicillin-resistant Staphylococcus aureus.

Author information

1
Department of Microbiology, Saaii College of Medical Science and Technology, Chaubepur, Kanpur 209203, India.
2
Microbiology and Microbial Biotechnology Laboratory, Department of Botany, University of Gour Banga, Malda, West Bengal 732103, India. Electronic address: smajumdarwbes@gmail.com.

Abstract

OBJECTIVE:

This study examined the antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava against methicillin-resistant Staphylococcus aureus (MRSA) and used a standardized purification protocol to determine the presence and abundance of bioactive compounds in the leaf extracts.

METHODS:

In vitro antimicrobial activities of the ethanolic extracts of C. sativa, T. orientalis and P. guajava were tested against MRSA. The presence of bioactive molecules in these three leaves was evaluated using biochemical assays and high-performance thin-layer chromatography (HPTLC).

RESULTS:

Resistance to methicillin, penicillin, oxacillin and cefoxitin was observed in each of the clinical and nonclinical MRSA isolates. However, they were still vulnerable to vancomycin. Used individually, the 50% extract of each plant leaf inhibited MRSA growth. A profound synergism was observed when C. sativa was used in combination with T. orientalis (1:1) and when P. guajava was used in combination with T. orientalis (1:1). This was shown by larger zones of inhibition. This synergism was probably due to the combined inhibitory effect of phenolics present in the leaf extracts (i.e., quercetin and gallic acid) and catechin, as detected by HPTLC.

CONCLUSION:

The leaf extracts of C. sativa, T. orientalis and P. guajava had potential for the control of both hospital- and community-acquired MRSA. Moreover, the inhibitory effect was enhanced when extracts were used in combination.

KEYWORDS:

Antimicrobial activity; High-performance thin-layer chromatography; Methicillin-resistant Staphylococcus aureus; Plant extracts; Synergistic effect

PMID:
30120078
DOI:
10.1016/j.joim.2018.07.005
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center