Format

Send to

Choose Destination
Anal Chim Acta. 2018 Nov 15;1031:185-194. doi: 10.1016/j.aca.2018.05.020. Epub 2018 May 8.

Simultaneous determination of PUFA-derived pro-resolving metabolites and pathway markers using chiral chromatography and tandem mass spectrometry.

Author information

1
pharmazentrum frankfurt/ZAFES, Institute of Clinical Pharmacology, Goethe-University Frankfurt, Germany.
2
Institut des Biomolécules Max Mousseron, IBMM, UMR 5247, University of Montpellier, CNRS, ENSCM, Montepellier, France.
3
pharmazentrum frankfurt/ZAFES, Institute of Clinical Pharmacology, Goethe-University Frankfurt, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Project Group TMP, Frankfurt, Germany.
4
pharmazentrum frankfurt/ZAFES, Institute of Clinical Pharmacology, Goethe-University Frankfurt, Germany. Electronic address: ferreirosbouzas@em.uni-frankfurt.de.

Abstract

Lipid mediators play an important role as biological messengers involved in inflammatory processes. Deriving from different polyunsaturated fatty acids, endogenously built mediators featuring both pro- and anti-inflammatory properties as well as pro-resolving lipid mediators and their biological precursors have been investigated. A newly developed method using chiral chromatography-tandem mass spectrometry on human plasma has demonstrated its suitability for the simultaneous determination of prostaglandins, lipoxins, D-series derived resolvins as well as protectins, maresin 1, leukotriene B4 and several precursors of them in order to yield information about metabolic pathways. Due to the matrix complexity, a solid phase extraction method using an octadecyl-modified silica gel cartridge was carried out. The developed method allows the determination of 34 analytes in 25 min showing enough selectivity as well as precision and accuracy (≤15% relative standard deviation, ≤15% relative error) in the calibration range of 0.1-10 ng mL-1 or 0.2-20 ng mL-1 depending on the analytes. Stability of the analytes in plasma has been demonstrated for at least 3 h at room temperature, 72 h in the autosampler and 60 days in the freezer at -80 °C. This method has been validated and shown its suitability for the determination of all studied analytes in human plasma samples.

KEYWORDS:

Chiral chromatography; LC-MS/MS; Resolution; Sensitivity; Specialized pro-resolving lipid mediators

PMID:
30119738
DOI:
10.1016/j.aca.2018.05.020
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center