Format

Send to

Choose Destination
Biomed Pharmacother. 2018 Oct;106:1506-1512. doi: 10.1016/j.biopha.2018.07.065. Epub 2018 Jul 24.

Novel endodontic sealers induced satisfactory tissue response in mice.

Author information

1
Department of Pediatric Dentistry, University of São Paulo, School of Dentistry of Ribeirão Preto, Brazil. Electronic address: lea@forp.usp.br.
2
Department of Pediatric Dentistry, University of São Paulo, School of Dentistry of Ribeirão Preto, Brazil. Electronic address: amanda.bertasso@usp.br.
3
Department of Pediatric Dentistry, University of São Paulo, School of Dentistry of Ribeirão Preto, Brazil. Electronic address: carolinamaschietto@yahoo.com.br.
4
Department of Pediatric Dentistry, University of São Paulo, School of Dentistry of Ribeirão Preto, Brazil. Electronic address: raquel@forp.usp.br.
5
Department of Dentistry of Lagarto, Federal University of Sergipe, Brazil. Electronic address: katharinamorant@hotmail.com.
6
Department of Restorative Dentistry, University of São Paulo, School of Dentistry of Ribeirão Preto, Brazil. Electronic address: sousanet@forp.usp.br.
7
Department of Pathology, University of São Paulo, School of Dentistry of Bauru, Brazil. Electronic address: consolaro@uol.com.br.

Abstract

OBJECTIVES:

The aim of this study was to evaluate the subcutaneous response induced by Roeko Guttaflow2 (RG), Sealapex Xpress (SX), AH Plus (AHP) sealers.

METHODS:

100 BALB/c mice received implants in the subcutaneous tissue with the tested materials (10 animals per period for each evaluated sealer) and were evaluated after different experimental periods (7, 21 and 63 days), in each animal was placed a tube, the control group was an empty tube. Histological analysis evaluated semi-quantitatively the inflammatory infiltration, collagen fiber formation and tissue thickness. In addition, immunohistochemistry was performed for interleukin-6 (IL-6). Data were statistically analyzed (α = 0.05).

RESULTS:

RG promoted a greater collagen fiber formation at 7 days and 63 days compared to the CG (p = 0.004) and AHP (p = 0.005) respectively, while at 21 days, the SX promoted a greater reaction (p = 0.021). For the tissue thickness, there was a greater reaction at 7 days with CG (p = 0.0156) and with RG at 63 days (p = 0.03). Regarding the inflammatory infiltrate, there was no difference at 7 days and 63 days (p = 0.5; p = 0.27), while at 21 days, a statistically difference was found between SX, CG (p = 0.04) and RG (p = 0.027). In addition, the presence of IL-6 was observed in almost all groups, with a more intense marking at 7days.

SIGNIFICANCE:

All cements evaluated presented a satisfactory tissue response, however, RG was the one that presented a more satisfactory tissue response.

KEYWORDS:

AHPlus; Endodontic sealers; Roeko Guttaflow2; Sealapex xpress; Subcutaneous tissue

PMID:
30119226
DOI:
10.1016/j.biopha.2018.07.065
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center