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Elife. 2018 Aug 17;7. pii: e37166. doi: 10.7554/eLife.37166.

MAPLE (modular automated platform for large-scale experiments), a robot for integrated organism-handling and phenotyping.

Author information

1
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, United States.
2
Center for Brain Science, Harvard University, Cambridge, United States.
3
Planetary Health Alliance, Harvard University, Cambridge, United States.
4
FlySorter LLC, Seattle, United States.

Abstract

Lab organisms are valuable in part because of large-scale experiments like screens, but performing such experiments over long time periods by hand is arduous and error-prone. Organism-handling robots could revolutionize large-scale experiments in the way that liquid-handling robots accelerated molecular biology. We developed a modular automated platform for large-scale experiments (MAPLE), an organism-handling robot capable of conducting lab tasks and experiments, and then deployed it to conduct common experiments in Saccharomyces cerevisiae, Caenorhabditis elegans, Physarum polycephalum, Bombus impatiens, and Drosophila melanogaster. Focusing on fruit flies, we developed a suite of experimental modules that permitted the automated collection of virgin females and execution of an intricate and laborious social behavior experiment. We discovered that (1) pairs of flies exhibit persistent idiosyncrasies in social behavior, which (2) require olfaction and vision, and (3) social interaction network structure is stable over days. These diverse examples demonstrate MAPLE's versatility for automating experimental biology.

KEYWORDS:

C. elegans; D. melanogaster; S. cerevisiae; automation; bombus impatiens; culturing; experimental robot; high throughput phenotyping; neuroscience; physarum polycephalum

PMID:
30117804
PMCID:
PMC6193762
DOI:
10.7554/eLife.37166
[Indexed for MEDLINE]
Free PMC Article

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