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Int J Syst Evol Microbiol. 2018 Oct;68(10):3212-3216. doi: 10.1099/ijsem.0.002963. Epub 2018 Aug 17.

Acinetobacter wuhouensis sp. nov., isolated from hospital sewage.

Author information

1
1​Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, PR China.
2
2​Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, PR China.
3
3​Laboratory of Bacterial Genetics, Centre for Epidemiology and Microbiology, National Institute of Public Health, Šrobárova 48, 100 42 Prague 10, Czech Republic.
4
4​Department of Laboratory Medicine, Third Faculty of Medicine, Charles University, Šrobárova 50, 100 34 Praha 10, Czech Republic.
5
5​Center for Pathogen Research, West China Hospital, Sichuan University, Chengdu, PR China.

Abstract

We recovered eight strains of the genus Acinetobacter from hospital sewage at West China Hospital in Chengdu, China. Based on the comparative analysis of the rpoB sequence, these strains formed a strongly supported and internally coherent cluster (intra-cluster identity of ≥98.0 %), which was clearly separated from all known Acinetobacter species (≤91.1 %). The eight strains also formed a tight and distinct cluster based on the genus-wide comparison of whole-cell mass fingerprints generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. In addition, the combination of their ability to assimilate 2,3-butanediol and phenylacetate, but not 4-hydroxybenzoate, and the inability to grow at 37 °C could distinguish these eight strains from all known Acinetobacter species. Whole-genomic sequencing has been performed for two selected strains, WCHA60T and WCHA62. There were 96.65 % average nucleotide identity (ANI) and 72 % in silico DNA-DNA hybridization (isDDH) values between WCHA60T and WCHA62, suggesting that the two strains indeed belonged to the same species. In contrast, the ANI and isDDH values between the two strains and the known Acinetobacter species were <83 and <30 %, respectively; both of which were far below the cut-off to define a bacterial species. Therefore, the eight strains should be considered to represent a novel species of the genus Acinetobacter, for which the name Acinetobacterwuhouensis sp. nov. is proposed. The type strain is WCHA60T (=CCTCC AB 2016204T=GDMCC 1.1100T=KCTC 52505T).

KEYWORDS:

MALDI-TOF MS; metabolic properties; multilocus sequence analysis

PMID:
30117799
DOI:
10.1099/ijsem.0.002963
[Indexed for MEDLINE]

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