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Dose Response. 2018 Aug 9;16(3):1559325818790158. doi: 10.1177/1559325818790158. eCollection 2018 Jul-Sep.

Role of N-Acetylcysteine and Coenzyme Q10 in the Amelioration of Myocardial Energy Expenditure and Oxidative Stress, Induced by Carbon Tetrachloride Intoxication in Rats.

Author information

1
Department of Pharmacology, King Saud University, Riyadh, Saudi Arabia.
2
Department of Pharmacology, Al-Azhar University, Cairo, Egypt.
3
Department of Pharmacology and Toxicology, Suez Canal University, Ismailia, Egypt.
4
Department of Zoology, King Saud University, Riyadh, Saudi Arabia.
5
Electron Microscope and Thin Films Department, National Research Center, Cairo, Egypt.
6
Department of Genetics and Cytology, National Research Center, Cairo, Egypt.
7
King Saud University, Riyadh, Saudi Arabia.

Abstract

This study is designed to evaluate the potential impact of N-acetyl cysteine (NAC) and coenzyme Q10 (CoQ10) each alone or in combination against carbon tetrachloride (CCl4)-induced cardiac damage in rats. Animals were treated with CCl4 in single intraperitoneal dose of 1 mL/Kg body weight; CCl4-intoxicated animals were pretreated with 20 mg/kg/d NAC or pretreated with 200 mg/kg/d CoQ10 or NAC and CoQ10 with the same previously mentioned doses. Carbon tetrachloride-intoxicated rats showed a significant elevation in nitric oxide and lipid peroxides and downregulation in reduced glutathione level and calcium adenosine triphosphatase. Cardiac glycolytic enzymes levels such as lactate dehydrogenase, phosphofructokinase, and hexokinase were declined coupled with a reduction in glucose content after CCl4 treatment. Moreover, myocardial hydroxyproline level was significantly increased after CCl4-treatment indicating accumulation of interstitial collagen. N-acetyl cysteine and/or CoQ10 effectively alleviated the disturbances in myocardial oxidative stress and antioxidant markers. These antioxidants effectively upregulated the reduction in cardiac energetic biomarkers due to CCl4 treatment. N-acetyl cysteine and/or CoQ10 significantly decreased hydroxyproline level compared to that of CCl4-treated rats. The current data showed that the aforementioned antioxidants have a remarkable cardioprotective effect, suggesting that they may be useful as prophylactic agents against the detrimental effects of cardiotoxins.

KEYWORDS:

calcium adenosine triphosphatase; glycolytic enzymes; hexokinase; lactate dehydrogenase; phosphofructokinase

Conflict of interest statement

Declaration of Conflicting Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

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