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Gene. 1986;41(2-3):321-5.

Cloning and characterization of the Schizosaccharomyces pombe DNA ligase gene CDC17.

Abstract

The Schizosaccharomyces pombe CDC17 gene has been cloned by complementation of the cdc17 mutant coding for temperature-sensitive DNA ligase. An allele-specific suppressor active only in the presence of a high osmotic pressure was also isolated. The cloned CDC17 gene failed to complement the analogous DNA ligase mutation, cdc9, in Saccharomyces cerevisiae, although the reverse complementation was successful [Barker and Johnston, Eur. J. Biochem. 134 (1983) 315-319]. The CDC17 gene specifies a 2.8-kb transcript.

PMID:
3011605
DOI:
10.1016/0378-1119(86)90114-9
[Indexed for MEDLINE]

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