Format

Send to

Choose Destination
Nat Commun. 2018 Aug 16;9(1):3279. doi: 10.1038/s41467-018-05793-2.

The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner.

Author information

1
Centre for Medical Parasitology at Department of Immunology and Microbiology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200, Copenhagen, Denmark.
2
Vancouver Prostate Centre, Vancouver, BC, V6H 3Z6, Canada.
3
Department of Urologic Sciences, University of British Columbia, Vancouver, BC, V5Z 1M9, Canada.
4
Stem Cells in Cancer & Ageing, Barts Cancer Institute, Queen Mary University of London, London, EC1M 6BQ, United Kingdom.
5
Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, 11490, Taipei, Taiwan.
6
Department of Molecular Oncology, British Columbia Cancer Research Centre, Vancouver, BC, V5Z 1L3, Canada.
7
UCL Cancer Institute, University College London, London, WC1E 6BT, United Kingdom.
8
Department of Medical Oncology, Barts Health NHS, London, EC1A 7BE, United Kingdom.
9
Stem Cells in Cancer & Ageing, Barts Cancer Institute, Queen Mary University of London, London, EC1M 6BQ, United Kingdom. c.heeschen@unsw.edu.au.
10
School of Medical Sciences, University of New South Wales, Sydney, NSW, 2052, Australia. c.heeschen@unsw.edu.au.
11
Centre for Medical Parasitology at Department of Immunology and Microbiology, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200, Copenhagen, Denmark. salanti@sund.ku.dk.

Abstract

Isolation of metastatic circulating tumor cells (CTCs) from cancer patients is of high value for disease monitoring and molecular characterization. Despite the development of many new CTC isolation platforms in the last decade, their isolation and detection has remained a challenge due to the lack of specific and sensitive markers. In this feasibility study, we present a method for CTC isolation based on the specific binding of the malaria rVAR2 protein to oncofetal chondroitin sulfate (ofCS). We show that rVAR2 efficiently captures CTCs from hepatic, lung, pancreatic, and prostate carcinoma patients with minimal contamination of peripheral blood mononuclear cells. Expression of ofCS is present on epithelial and mesenchymal cancer cells and is equally preserved during epithelial-mesenchymal transition of cancer cells. In 25 stage I-IV prostate cancer patient samples, CTC enumeration significantly correlates with disease stage. Lastly, rVAR2 targets a larger and more diverse population of CTCs compared to anti-EpCAM strategies.

PMID:
30115931
PMCID:
PMC6095877
DOI:
10.1038/s41467-018-05793-2
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center