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J Proteome Res. 2018 Dec 7;17(12):4320-4324. doi: 10.1021/acs.jproteome.8b00408. Epub 2018 Aug 20.

Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry.

Hwang H1,2,3, Jeong JE1,2, Lee HK1,2, Yun KN1,4, An HJ2,3, Lee B5,6, Paik YK7, Jeong TS8, Yee GT8, Kim JY1, Yoo JS1,2.

Author information

1
Biomedical Omics Research , Korea Basic Science Institute , Cheongju , Korea.
2
Graduate School of Analytical Science and Technology , Chungnam National University , Daejeon , Korea.
3
Asia-Pacific Glycomics Reference Site , Chungnam National University , Daejeon , Korea.
4
Department of Chemistry , Sogang University , Seoul , Korea.
5
Department of Anatomy & Cell Biology, Graduate School of Medicine , Gachon University , Incheon , Korea.
6
Center for Genomics and Proteomics & Stem Cell Core Facility, Lee Gil Ya Cancer and Diabetes Institute , Gachon University , Incheon , Korea.
7
Yonsei Proteome Research Center and Department of Integrated OMICS for Biomedical Science, and Department of Biochemistry, College of Life Science and Biotechnology , Yonsei University , Seoul , Korea.
8
Department of Neurosurgery , Gil Medical Center, Gachon University , Incheon , Korea.

Abstract

We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a < 1.0% false discovery rate at the peptide and protein levels, we identified 3731 proteins, among which five were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. No olfactory receptor (OR) proteins were detected in olfactory tissue, suggesting that detection of ORs would be very difficult. We also identified 49 and 50 alternative splicing variants mapped at the neXtProt and GENCODE databases, respectively, and 2000 additional single amino acid variants. This data set is available at the ProteomeXchange consortium via PRIDE repository (PXD010025).

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